Aplikasi Kultur Jaringan dalam Pemeliharaan Bibit Rumput Laut Kappaphycus alvarezii di Laboratorium BPBL Ambon

Meillisa Carlen Mainassy; Asinado Hutabalian1; Madellayne Glosallya Ririmasse; Meylany Songjanan

doi:10.65055/bhaktijivana.v2i2.40

Authors

Meillisa Carlen Mainassy Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Pattimura
Asinado Hutabalian Program Studi Biologi, Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Pattimura
Madellayne Glosallya Ririmasse Program Studi Biologi, Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Pattimura
Meylany Songjanan Program Studi Biologi, Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Pattimura

DOI:

https://doi.org/10.65055/bhaktijivana.v2i2.40

Keywords:

Tissue culture application, Laboratory, Kappaphycus alvarezii, Seaweed

Abstract

This community service activity aimed to apply tissue culture techniques for developing high-quality seedlings of Kappaphycus alvarezii (Cotoni seaweed) at the Marine Aquaculture Center (BPBL) Laboratory in Ambon. The methods involved the preparation of solid and liquid PES-based media, sterilization of tools and materials, and maintenance of micropropagules and plantlets under aseptic conditions. Observations focused on media quality, plantlet growth, and subculture frequency. Results showed that P2.5NW1PW1T and SM FITO media supported optimal plantlet development with low mortality rates, while media with high hormone concentrations induced plantlet death. The findings emphasize the importance of media formulation and water quality monitoring in tissue culture success. This program demonstrated a practical contribution to the sustainable cultivation technology of seaweed aquaculture.